There is no margin for error in flow cytometry
The quality of the analytical measurement data depends entirely on the stability of this liquid flow. Fluctuations in the flow rate directly affect the exact position of the cell in the measurement zone and compromise repeatability. For high-resolution cell analysis, a stable, low-pulsation sheath flow of 1 to 10 ml/min, low dead volumes and extremely precise fluid handling are therefore of crucial importance.
The development of high-quality flow cytometers places stringent and specific demands on the integrated fluidic system. This applies both to the supply of the sheath fluid and to the pumping of the cell and particle suspensions themselves.
Key requirements for the fluid system:
- Sstable sheath flow (1 to 10 ml/min): Constant flow conditions for reproducible measurement results, even over long cycle periods.
- Pulsation-free dosing: Prevents turbulence and fluctuations in the microfluidic channels.
- Gentle handling of samples: Low shear forces protect vulnerable cells and particles from damage.
- Minimum dead volume: Enables efficient medium changes and reduces the consumption of expensive reagents.
- Compact integration: Provides a solution for integration into compact OEM analytical instruments.
- Chemical resistance: Suitable for regular cleaning and chemical sterilisation procedures.
A stable fluidic process makes a direct contribution to the overall measurement quality and data reliability in high-resolution cell analysis.
